UV-LED Induction of Measurable Biological Endpoints

ABSTRACT

Composition, systems, and methods for the induction of biological endpoints in a cheap contained system. In many embodiments, variation on well numbers on plates and ranging ultraviolet wavelengths produces different and controlled outcomes of the biological endpoint induction. Endpoint induction of this type allows for chemical induction, wherein the system can be turned on and off in short time periods.

FEDERAL FUNDS STATEMENT

This invention was made with Government support under SBIR/STTR contractnumber ES032435. The Government has certain rights in this invention.

TECHNICAL FIELD

Embodiments of the present invention relate to inducing measurablebiological endpoints. More particularly embodiments may allow for studyin organic responses to stress.

PRIOR ART

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BACKGROUND

Standard stress induction systems are bulky not easily portable and donot utilize the latest techniques. Utilizing new light emitting diodesallows for more portable and smaller batch applications. These inductionsystems are either radiation-based system available to hospitals andlarge research institutions, or chemical induction, which is messy andleaves residue on the cells, not amenable to repair based assays.

One major innovation of the technology is the precision of the system,where using chemical induction, the chemical needs to be placed on thecells, incubated, taken off the cells, cells washed multiple times, butchemical residue remains. The induction system, through an optogeneticapproach, is instantaneous and can be turned on and off in milliseconds.

BRIEF DESCRIPTION OF THE DRAWINGS

The various advantages of the embodiments of the present invention willbecome apparent to one skilled in the art by reading the followingspecification and appended claims, and by referencing the followingdrawings, in which:

FIG. 1 is a pictorial representation of an example of a compositionaccording to an embodiment;

FIG. 2 is a diagrammatic representation of an example of a systeminducing biological endpoints according to an embodiment; and

DETAILED DESCRIPTION

Turning to FIG. 1 the composite parts in an embodiment include a wellplate component 10, a light cone array component 12, and ultravioletlight-emitting diode component 14. These three components may becombined to create the damage induction system 16.

In another embodiment, the damage induction system 16 may be modified byadding additional wells or reducing number of wells in component 10.

In many embodiments, the damage induction system 16 acts as anoptogenetic approach.

In many embodiments, the wells 10 might number from one large well to1536 microwells.

In one embodiment of the damage induction system 16, the ultravioletlight emitting diode component 14 selected might use nanometer lengths220 to 400. These wavelengths inducing different measurable endpoints.

In another embodiment, well plate component 10 might be removable.

In one embodiment light emitting diode component 14 might modulate thewavelengths used based on an algorithm component 18.

In one embodiment light cone array component 12, sample well platecomponent 10, and light emitting diode component 14 combines into aportable damage induction system 16.

In one embodiment system might include a power source component 20; thiscomponent might be standard commercial transformer. This or anothersystem might include a heat sink component 22.

In one embodiment plate component 10 might be composed of plate-basedirradiation spectrophotometer curvettes.

In one embodiment plate component 10 might be composed of 15 ml and 50ml tubes.

Many embodiments might involve the illustrated components beinginstalled into a larger sample analysis system. Where the end result isa damage induction system 16. The components ingredients well platecomponent 10, light cone array component 12, and light emitting diodecomponent 14 may also be included in a large device, so it is possiblethat making biological endpoints result in further analysis.

In one embodiment the system has an onboard cooling component thatallows the unit to be placed in a biological incubator to be able toirradiate biological material at temperature so as to not disturb thecell environment.

One embodiment might utilize an algorithm 28 determining exposure andtiming for induction. In some embodiments FIG. 2 could result inmodulating wavelengths to produce measurable endpoints. Once theinduction system 26 is controlled by an algorithm 28 as it might in oneexample of the schema, it can result in varied samples 30 for analysis.

In many embodiments of the induction system 26, samples 30 might bederived from the addition of algorithm 28 and exposure modulationcomponent 24.

In some embodiments the induction system 26 does not utilize algorithm28 or exposure modulation component 24. In the schema the system mightinclude only the algorithm 28 in addition with the induction system 26;or alternatively only the exposure modulation component 24 with theinduction system 26.

RELATED APPLICATIONS

The present application claims priority to and benefit from U.S.Provisional Application No. 63/270,892 filed Oct. 22, 2021, the entirecontents of each of which are herein incorporated by reference.

We claim:
 1. A method of inducing measurable biological endpoints,including genetic manipulation by providing light at a wavelength withina range of 220 nanometer to 400 nanometers, wherein light source is alight emitting diode.
 2. The method as in claim 1, wherein the lightwavelength range is 340 nanometers to 400 nanometers.
 3. The method asin claim 1, wherein the light wavelength range is 310 nanometers to 340nanometers.
 4. The method as in claim 1, wherein the light wavelengthrange is 280 nanometers to 310 nanometers.
 5. The method as in claim 1,wherein the light wavelength range is 220 nanometers to 280 nanometers.6. A system comprising: a light emitting diode component; a specimenwell plate component; and an exposure control component.
 7. The systemas in claim 6, wherein the specimen well plate component is removable.8. The system as in claim 6, wherein the well plate component isvariable in size up to 1536 wells.
 9. The method as in claim 6, whereineach well plate contains a different sample in each well.
 10. The systemas in claim 6, wherein the well plate component are spectrophotometercurvettes.
 11. The system as in claim 6, wherein the light emittingdiode component emits light in wavelength range 340 nanometers to 400nanometers.
 12. The system as in claim 6, wherein the light emittingdiode component emits light in wavelength range 310 nanometers to 340nanometers.
 13. The system as in claim 6, wherein the light emittingdiode component emits light in wavelength range 280 nanometers to 310nanometers.
 14. The system as in claim 6, wherein the light emittingdiode component emits light in wavelength range 220 nanometers to 280nanometers.
 15. A method of making measurable biological endpoints,including genetic manipulation comprising: providing a sample; addinglight from light emitting diode light source; and observing result. 16.The method as in claim 15, further comprising adding a well plate. 17.The method as in claim 16, wherein well plate is removable.
 18. Themethod as in claim 16, wherein well plate is variable in size up to 1536wells.
 19. The method as in claim 16, wherein each well plate contains adifferent sample in each well.
 20. The method as in claim 16, whereinthe well plate component are spectrophotometer curvettes.
 21. The methodas in claim 15, wherein the light emitting diode component emits lightin wavelength range 340 nanometers to 400 nanometers.
 22. The method asin claim 15, wherein the light emitting diode component emits light inwavelength range 310 nanometers to 340 nanometers.
 23. The method as inclaim 15, wherein the light emitting diode component emits light inwavelength range 280 nanometers to 310 nanometers.
 24. The method as inclaim 15, wherein the light emitting diode component emits light inwavelength range 220 nanometers to 280 nanometers.